Hey everyone, so it's the first time I'm attempting to make a tile set for a game and I'm not sure what things should go into consideration for making it, right now I've just been working with standard units of 100 pixels by a 100 pixels and slowly constructing various pieces from there. Does any one have any advice for me on how to avoid any future headache before I get too far with this? I'd normally make this in 3D but I'd like to try 2D level construction for a change; I will also be making the game levels in Unity.
Below is a sample of a few tiles so far:

Thanks to everyone for their time.